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Polyphenol Platform


Head : Dr. Véronique Cheynier, HDR
   Senior Scientist
   Phone: + 33 (0)4 99 61 22 98
   Email :

Because of the structural diversity, characterisation of phenolic compounds in plants and plant based food and non food products,  requires an integrated analytical approach. The polyphenol platform thus offers analytical, methodological and technical help, as well as expertise in mass spectrometry, nuclear magnetic resonance and chemometrics for :

·     structural characterisation
·     quantification
·     high throughput analysis of large series of samples

The Polyphenol platform is a labelled analytical facility since 2003: GIS IBiSA (Infrastructures en Biologie Santé et Agronomie), CNOC-INRA and GPTR.


The Polyphenol platform hosts three divisions :
·    NMR : Dr. Christine Le Guernévé
·    Mass spectrometry : Dr. Nicolas Sommerer, Mrs. Emmanuelle Meudec and Mr. Arnaud Verbaere
·    Chemometrics : Dr. Jean-Claude Boulet

Request for analysis

For each request,  please fill the corresponding request form.
Request form for NMR analysis
Request form for mass spectrometry analysis

A quote is sent after reception of the request form.

Nuclear Magnetic Resonance (NMR)


Contact : Dr. Christine Le Guernevé
   Research Engineer INRA
   Phone : + 33 (0)4 99 61 28 58
   Email :


NMR spectrometer VARIAN Unity-Inova 500 MHz (11.57 Tesla)

4 probes
A 10mm broad band probe (31P-109Ag), a 5mm switchable probe (1H/19F and 31P-109Ag), and a double resonance 3mm inverse probe for
·    10 mm Broad-Band probe  (31P - 109Ag)
·    5 mm switchable probe ( 1H/19F and 31P - 109Ag)
·    a double resonance 3 mm probe, equipped with a z-axis gradient, for 1H observation and 15N/13C decoupling.
·    a double resonance 5 mm probe, equipped with a z-axis gradient, for 1H observation and 15N/13C decoupling

·    1D experiments 1H, 19F and X (31P-109Ag) ; 1H /X decoupling; X/1H decoupling
·    1D multi-impulsion experiments (DETP, INEPT, mesure de T1 et de T2)
·    Homonoclear 2D experiments 1H-1H : COSY, TOCSY, NOESY, ROESY
·    Heteronuclear 2D experiments 1H-X : HMQC, HSQC, HMBC
·    Relative and absolute quantification of compounds in mixtures

Mass spectrometry


Contact : Dr. Nicolas Sommerer, HDR
   Research Engineer INRA
   Phone : + 33 (0)4 99 61 25 84
   Email :


2 mass spectrometers coupled to UHPLC to perform the 3 types of analyses. All of them are equipped with UV-visible diode array detectors and of ESI and APCI ionisation sources.  

Structural characterisation:

UHPLC-MSn (Waters Acquity UPLC - Bruker AmaZonX 3D ion trap)

Quantification and high throughput targeted analysis:

UHPLC- triple quadrupole mass spectrometer (Waters Acquity UPLC - TQD)



Contact : Dr. Jean Claude Boulet
   Research Engineer INRA
   Phone : + 33 (0)4 99 61 31 48
   Email :


Chemometrics includes a range of data analysis techniques enabling to build stochastic models in order to predict one or more characteristics of the studied samples, starting from their spectra (MS, NMR, UV-visible, IR). Demands regarding such studies, requiring acquisition of a spectral library, must be examined by the platform committee.


SAS (Statistical Analysis System)
Scilab (a free mathematics software) with the module FACT (Free Access Chemometric Toolbox) developed and maintained by the polyphenol analysis platform.

Recent publications (2012-2017) :

Ferreira-Lima N, Vallverdú-Queralt A, Meudec E, Pinasseau L, Verbaere A, Bordignon-Luiz MT, Le Guernevé C, Cheynier V, Sommerer N
Quantification of hydroxycinnamic derivatives in wines by UHPLC-MRM-MS
Analytical and Bioanalytical Chemistry, sous presse

Carrascón V, Vallverdú-Queralt A, Meudec E, Sommerer N, Fernandez-Zurbano P, Ferreira V
The kinetics of oxygen and SO2 consumption by red wines. What do they tell about oxidation mechanisms and about changes in wine composition?
Food Chemistry, 2018, 206-214  doi: 10.1016/j.foodchem.2017.08.090

Pinasseau L, Vallverdú-Queralt A, Verbaere A, Roques M, Meudec E, Le Cunff L, Peros JP, Ageorges A, Sommerer N, Boulet JC, Terrier N, Cheynier V
Cultivar diversity of grape skin polyphenol composition and changes in response to drought investigated by LC-MS based metabolomics
Frontiers in Plant Science, 2017, 8, 1826  doi: 10.3389/fpls.2017.01826

Vallverdú-Queralt A, Meudec E, Eder M, Lamuela-Raventos RM, Sommerer N, Cheynier V
The Hidden Face of Wine Polyphenol Polymerization Highlighted by High-Resolution Mass Spectrometry
ChemistryOpen, 2017, doi: 10.1002/open.201700044 (open access article)

Vallverdú-Queralt A, Meudec E, Eder M, Lamuela-Raventos RM, Sommerer N, Cheynier V
Targeted filtering reduces the complexity of UHPLC-Orbitrap-HRMS data to decipher polyphenol polymerization
Food Chemistry, 2017, 255-263 doi:10.1016/j.foodchem.2017.01.106

Pinasseau L, Verbaere A, Roques M, Meudec E, Vallverdú-Queralt A, Terrier N, Boulet JC, Cheynier V,  Sommerer N
A Fast and Robust UHPLC-MRM-MS Method to Characterize and Quantify Grape Skin Tannins after Chemical Depolymerization
Molecules, 2016, 21(10), 1409 doi:10.3390/molecules21101409

Ferreira-Lima N, Vallverdú-Queralt A, Meudec E, Mazauric J-P, Sommerer N, Bordignon-Luiz MT, Cheynier V, Le Guernevé C
Synthesis, Identification, and Structure Elucidation of Adducts Formed by Reactions of Hydroxycinnamic Acids with Glutathione or Cysteinylglycine
Journal of Natural Products, 2016, 2211-2222 doi:10.1021/acs.jnatprod.6b00279

Bontpart T, Marlin T, Vialet S, Guiraud JL, Pinasseau L, Meudec E, Sommerer N, Cheynier V, Terrier N
Two shikimate dehydrogenases, VvSDH3 1 and VvSDH4, are involved in gallic acid biosynthesis in grapevine
Journal of Experimental Botany, 2016, 3537-3550 doi:10.1093/jxb/erw184

Marone A, Carmona-Martínez AA, Sireb Y, Meudec E, Steyer JP, Bernet N, Trably E
Bioelectrochemical treatment of table olive brine processing wastewater for biogas production and phenolic compounds removal
Water Research, 2016, 316-325 doi:10.1016/j.watres.2016.05.008

Vallverdu-Queralt A, Meudec E, Ferreira-Lima N, Sommerer N, Dangles O, Cheynier V, Le Guernevé C
A comprehensive investigation of guaiacyl-pyranoanthocyanin synthesis by one-/two-dimensional NMR and UPLC-DAD-ESI-MSn
Food Chemistry, 2016, 902-910 doi:10.1016/j.foodchem.2015.12.089

Pineda-Vadillo C, Nau F, Guerin Dubiard C, Cheynier V, Meudec E, Sanz-Buenhombre M, Guadarrama A, Tóth T, Csavajda E, Hingyi H, Karakaya S, Sibakov J, Capozzi F, Bordoni A, Dupont D
In vitro digestion of dairy and egg products enriched with grape extracts: Effect of the food matrix on polyphenol bioaccessibility and antioxidant activity
Food Research International, 2016, 284-292 doi:10.1016/j.foodres.2016.01.029

Lambert M, Meudec E, Verbaere A, Mazerolles G, Wirth J, Masson G, Cheynier V, Sommerer N
A high-throughput UHPLC-QqQ-MS method for polyphenol profiling in rosé wines
Molecules, 2015, 7890-7914 doi:10.3390/molecules20057890  / download pdf (open access article)

Vallverdu-Queralt A, Verbaere A, Meudec E, Cheynier V, Sommerer N
Straightforward method to quantify GSH, GSSG, GRP and hydroxycinnamic acids in wines by UPLC-MRM-MS
Journal of Agricultural and Food Chemistry, 2015, 142−149 doi:10.1021/jf504383g

Doco T, Williams P, Meudec E, Cheynier V, Sommerer N
Complex carbohydrates of red wine: Characterization of the extreme diversity of neutral oligosaccharides by ESI-MS
Journal of Agricultural and Food Chemistry, 2015, 671-682 doi:10.1021/jf504795g

Boulet JC, Trarieux C, Souquet JM, Ducasse MA, Caillé S, Samson A, Williams P, Doco T, Cheynier V
Models based on ultraviolet spectroscopy, polyphenols, oligosaccharides and polysaccharides for prediction of wine astringency
Food Chemistry, 2015, doi:10.1016/j.foodchem.2015.05.062

Koffi EN, Meudec E, Adjé FA, Lozano PR, Lozano YF, Bekro YA
Effect of reverse osmosis concentration coupled with drying processes on polyphenols and antioxidant activity obtained from Tectona grandis leaf aqueous extracts
Journal of Applied Research on Medicinal and Aromatic Plants, 2015, 54-59 doi:10.1016/j.jarmap.2015.03.001

Pérez-Mañá C, Farré M, Rodríguez-Morató J, Papaseit E, Pujadas M, Fitó M, Robledo P, Covas MI, Cheynier V, Meudec E, Escudier JL, de la Torre R
Moderate consumption of wine, through both its phenolic compounds and alcohol content, promotes hydroxytyrosol endogenous generation in humans. A randomized controlled trial.
Molecular Nutrition & Food Research, 2015, 1213-1316 doi:10.1002/mnfr.201400842

Cheynier V, Tomas-Barberan FA, Yoshida K
Polyphenols: From Plants to a Variety of Food and Nonfood Uses
Journal of Agricultural and Food Chemistry, 2015, 7589-7594 doi: 10.1021/acs.jafc.5b01173

Martin JC, Maillot M, Mazerolles G and 35 others.
Can we trust untargeted metabolomics? Results of the metabo-ring initiative, a large-scale, multi-instrument inter-laboratory study.
Metabolomics, 2015, 807-821 doi: 10.1007/s11306-014-0740-0

Mouls L, Mazauric JP, Hugouvieux V, Sommerer N, Mazerolles G, Fulcrand H
How to gain insight into the polydispersity of tannins: A combined MS and LC study
Food Chemistry, 2014, 348-353 doi:10.1016/j.foodchem.2014.05.121

Rezaire A,  Robinson JC, Bereau D, Verbaere A, Sommerer N, Khan MK,  Durand P,  Prost E, Fils-Lycaon B
Amazonian palm Oenocarpus bataua ("patawa"): chemical and biological antioxidant activity -phytochemical composition
Food Chemistry, 2014, 149 62–70 doi:10.1016/j.foodchem.2013.10.077

Habib HM, Platat C, Meudec E, Cheynier V, Ibrahim WH
Polyphenolic compounds in date fruit seed (Phoenix dactylifera): characterisation and quantification by using UPLC-DAD-ESI-MS
Journal of the Science of Food and Agriculture,2014, 1084-1089 doi:10.1002/jsfa.6387

Vetrani C, Rivellese AA, Annuzzi G, Mattila I, Meudec E, Hyotylainen T, Oresic M, Aura AM
Phenolic metabolites as compliance biomarker for polyphenol intake in a randomized controlled human intervention
Food Research International, 2014, 233-238 doi:10.1016/j.foodres.2014.01.018

Boulet JC, Brown SD, Roger JM
A simple, projection-based geometric model for several linear pretreatment and calibration methods
Chemometrics and Intelligent Laboratory Systems, 2014, 48-56 doi: 10.1016/j.chemolab.2014.07.012

Boulekbache-Makhlouf L, Meudec E, Mazauric JP, Madani K, Cheynier V
Qualitative and semi-quantitative analysis of phenolics in Eucalyptus globulus leaves by high-performance liquid chromatography coupled with diode array detection and electrospray ionisation mass spectrometry
Phytochemical Analysis, 2013, 162-70 doi: 10.1002/pca.2396

Koffi EN, Le Guernevé C, Lozano P, Meudec E, Adje FA, Bekro YA, Lozano YF
Polyphenol extraction and characterization of Justicia secunda Vahl leaves for traditional medicinal uses
Industrial Crops and Products, 2013, 49 : 682-689 doi:10.1016/j.indcrop.2013.06.001

Aouf C, Le Guernevé C, Caillol S, Fulcrand H
Study of the O-glycidylation of natural phenolic compounds. The relationship between the phenolic structure and the reaction mechanism
Tetrahedron, 2013, 1345-1353 doi: 10.1016/j.tet.2012.11.079

Boulet JC, Bertrand D, Mazerolles G, Sabatier R, Roger JM
A family of regression methods derived from standard PLSR
Chemometrics and Intelligent Laboratory Systems, 2013, 116-125 doi: 10.1016/j.chemolab.2012.11.002 download pdf

Boulet JC, Bertrand D, Roger JM
FACT: A Scilab Free Access Chemometric Toolbox (dépôt APP), 2013,

Boulet JC, Bendoula R, Roger JM, Ducournau P, Lagarde-Pascal C, Brenon E, Lieubeau V
Method and device for measuring a chemical or biological reaction state
Brevet Européen EP2836822, 2012European Patent Register

Adjé FA, Lozano YF, Le Guernevé C, Lozano P, Meudec E, Adima A, Gaydou E
Phenolic acid and flavonol water extracts of Delonix regia red flowers
Industrial Crops and Products, 2012, 303-310 doi: 10.1016/j.indcrop.2011.12.008

Cheynier V
Phenolic compounds: from plants to foods
Phytochemical Reviews, 2012, 153-177 doi: 10.1007/s11101-012-9242-8

Highlight on previous works :

Tarascou I, Souquet JM, Mazauric JP, Carrillo S, Coq S, Canon F, Fulcrand H, Cheynier V
The hidden face of food phenolic composition
Archives of Biochemistry and Biophysics, 2010, 16–22 doi:10.1016/

Mazerolles G, Preys S, Bouchut C, Meudec E, Fulcrand H, Souquet JM, Cheynier V
Combination of several mass spectrometry ionization modes: A multiblock analysis for a rapid characterization of the red wine polyphenolic composition
Analytica Chimica Acta, 2010, 195-202 doi:10.1016/j.aca.2010.07.034

Coq S, Souquet JM, Meudec E, Cheynier V, Hättenschwiler S
Interspecific variation in leaf litter tannins drives decomposition in a tropical rain forest of French Guiana
Ecology, 2010, 2080-2091 doi: 10.1890/09-1076.1

Fulcrand H, Mané C, Preys S, Mazerolles G, Bouchut C, Mazauric JP, Souquet JM, Meudec E, Li Y, Cole R, and Cheynier V
Direct mass spectrometry approaches to characterize polyphenol composition of complex samples
Phytochemistry, 2008, 3131-3138 doi:10.1016/j.phytochem.2008.03.016

Mané C, Sommerer N, Yalcin T, Cheynier V, Cole RB, Fulcrand H
Assessment of the molecular weight distribution of tannin fractions through MALDI-TOF MS analysis of protein-tannin complexes
Analytical Chemistry, 2007, 2239-2248 doi:10.1021/ac061685+

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